Head of Department

Dr. Róbert Horváth

Tel: +(36-1)-392 2696

E-mail: horvath.robert@ek-cer.hu

The Nanobiosensorics “Lendület” Group (www.nanobiosensorics.com) started its activity in July, 2012. The research profile is the development and application of label-free optical biosensors, the mathematical modeling of the relevant biological and biophysical processes.

The group made important steps both in the development of the research infrastructure and both in creating personal and collaborative network. The group had a successful collaboration with the Swiss startup, Creoptix AG on high resolution label-free optical sensing. Our closest collaboration partners are scientists from various Hungarian universities, such as Semmelweis University, Eötvös Lóránd University, Budapest University of Technology and Economics, and University of Pannonia.

We have established a cell culture laboratory, making possible to launch a new and promising research line, the “label-free biosensorics of living cells”. We have installed an EPIC multichannel biosensor prototype and a Holomonitor M4 holographic microscopy. It is important to note that both instruments are quite unique both nationally and internationally. We were among the first who could develop and apply these modern techniques. It is worth mentioning that we were the first who could measure the adhesion properties of microvesicles secreted by living cells and the surface adhesion kinetics of human monocytes using label-free optical biochips.

 The group focuses on the following main research topics; ranging form the kinetics of cellular adhesion, migration and signalling on novel biomimetic interfaces to the mathematical modelling of the measured biological signals.

Label-free biosensors and techniques. Our research is centered around different biosensing technologies; development and application of optical biosensors and fluidic techniques, plate-based methods and visualization. By combining some of the most powerful optical and mechanical methods we are capable of manipulating and sensing single cells, as well as populations with an exceptional accuracy. Our diverse technology park allows us to execute various measurements of biological and physical interest.

Biosensor coatings and characterization. Our laboratory has much experience in the production of polymers to influence cellular adhesion, for example dextran, PLL-g-PEG polymer and nanoparticle coatings on biosensor surfaces. We apply flagellin based biomimetic coatings as well to alter the adhesivity of the cells. Considering cancer cell adhesion and spreading the group first established a methodology to determine the integrin receptor-RGD ligand (cell adhesive motif of the PLL-g-PEG-RGD polymer) affinity constant in living cells without applying any labelling. Our results on measuring the structural order of nanometer scale biological layers (proteins, lipid bilayers) are similarly important.

Single cell analysis and manipulation. Single cell analysis is a new trend that makes it possible to discover mechanisms not seen when studying a bulk population of cells. Our label-free techniques are capable of measuring single cell adhesion force and other properties, and deposite single cells as well. The CellSorter automatized micropipette system is capable of sorting single cells with great accuracy and speed. Fluid FM is a truly unique combination of force microscopy and microfluidics elevates applications to a higher level, from single cell biology to surface analysis and beyond.

Compounds and stimuli. Label-free biosensors are capable of monitoring cellular responses to different compounds and stimuli. We have experience in for example B cell stimulation, gold nanoparticles and natural compound (green tea polyphenol, epigallocatechin-gallate) treatment of mammalian cells monitored by label-free techniques. The role of cellular glycocalyx in adhesion is also investigated by high resolution label-free methods.

Analytical and numerical modelling of biosensor data. Interpreatation and modelling of the received kinetic data is essential to understand the measured phenomena. Our team use for example Maple and Mathlab softwares to model biophysical processes.

In the mentioned topics, our laboratory could achieve strategic results, which were published in important international journals (Analytical Chemisty, Biosensors and Bioelectronics, Scientific Reports, Sensors and Actuators B, International Journal of Molecular Sciences, Langmuir, Colloids and Interfaces B, Plos One, Applied Physics Letters etc.), or are under submission.

These results can find further applications in biotechnology and medical diagnostics.

The website of the department: nanobisoensorics.com

The colleagues of the Department:

Dr. Róbert Horváth – Senior Research Fellow, Head of department

Dr. Inna Székács, Senior Research Fellow,

Dr. Sándor Kurunczi, Senior Research Fellow,

Dr. Beatrix Péter, Research Fellow

Dr. András Saftics, Research Fellow (currently in USA in postdoc position)

Dr. Zoltán Szittner, Research Fellow

Dr. Boglárka Kovács, Research Fellow (on maternity leave)

Dr. Rita Ungai-Salánki, Research Fellow (currently on ELTE in postdoc position)

Dr. Enikő Farkas, Junior Research Fellow

Tamás Gerecsei, PhD student

Ágoston Gábor Nagy, PhD student

Kinga Dóra Kovács, PhD student

Nicolett Kanyó, FIKU

Alexandra Vörös, FIKU

Milán Sztilkovics, MSc student

Péter Chrenkó, MSc student

Róbert Tarr, MSc student

Norbert Pap, MSc student

Beatrix Magyaródi, MSc student

Barbara Majoros, MSc student

Bence Joó, MSc student

Fatime Gajnut, MSc student

Kinga Tóth, BSc student